Calculating the melting temperature of PCR primers
SOLVED: Use the Wallace Rule equation to calculate the Tm of each of the primers you designed. Tm = 2(A+T) + 4(G+C) Left primer: 5' CCTGGACACTGTAC 3' Right primer: 5' AATGTATGAACCTG 3
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Primers for ITS1 region amplification and ISSR profiling. T m = Melting... | Download Scientific Diagram
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Primers for ISSR profiling. T m = Melting temperature of Primer, T A=... | Download Scientific Diagram
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SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
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